Journal :   Asian Journal of Research in Chemistry

Volume No. :   1

Issue No. :  1

Year :  2008

Pages :   36-39

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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HPTLC Method Development and Validation for the simultaneous Estimation of Diosgenin and Levodopa in marketed formulation



Address:   VB Kshirsagar*, UA Deokate, VB Bharkad and SS Khadabadi
Government College of Pharmacy, Amravati 444604 (M.S.).
*Corresponding Author
DOI No:

ABSTRACT:
The stationary phase silica gel G60F254 was selected for separation and the sample was developed using a mixture of Toluene: Ethyl acetate: Formic acid: GAA in the ratio 2:1:1: 0.75 v/v as mobile phase. Quantification was carried out at 194 nm for Diosgenin and 280 nm for Levodopa using absorbance reflectance mode. The Rf value of Levodopa and Diosgenin was found to be 0.27+0.2 and 0.61+0.2 respectively. Linearity was found to be in the concentration range of 100 to 700 ng/spot of Levodopa and 600 to 1800 for Diosgenin the correlation coefficient value is 0.9954 and 0.9934. The results of analysis were validated in terms of accuracy and precision. The LOD was found to be 1.03ng and 5.69ng of Levodopa and Diosgenin respectively. LOQ was found to be 3.14ng and 17.25ng/spot of Levodopa and Diosgenin respectively. The content uniformity test was carried out as per the USP specification. The proposed HPTLC method provides a faster and cost effective quantitative control for routine analysis of Levodopa and Diosgenin.
KEYWORDS:
Simultaneous estimation, Levodopa, Diosgenin, validation.
Cite:
VB Kshirsagar, UA Deokate, VB Bharkad, SS Khadabadi. HPTLC Method Development and Validation for the simultaneous Estimation of Diosgenin and Levodopa in marketed formulation. Asian J. Research Chem. 1(1): July-Sept. 2008;Page 36-39.
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