Journal :   Asian Journal of Research in Chemistry

Volume No. :   1

Issue No. :  2

Year :  2008

Pages :   88-90

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Reverse Phase High Performance Liquid Chromatographic Method for the Determination of Ethamsylate in Bulk and Tablet Dosage Forms



Address:   P Nagaraju, K Krishnachaithanya, VDN Srinivas, SVN Padma
Department of Pharmaceutical analysis, Hindu College of Pharmacy, Amaravathi Road, Guntur. India.
*Corresponding Author
DOI No:

ABSTRACT:
A simple, sensitive, precise and specific reverse phase high performance liquid chromatographic method was developed and validated for the determination of Ethamsylate in bulk and its tablet dosage forms. It was found that the excipient in the tablet dosage forms does not interfere in the quantification of active drug by proposed method. The HPLC separation was carried out by reverse phase chromatography on C18 column (4.6 x 250 mm, 5µm particle size) with a mobile phase composed of methanol: acetonitrile: acetate buffer PH 2.8 (60:30:10 v/v) in isocratic mode at a flow rate of 1 ml / min. The detection was monitored at 290 nm. The calibration curve for Ethamsylate was linear from 0.5 – 100 µg/ml. The interday and intraday precision (relative standard deviation) was found to be within the limits. The proposed method has adequate sensitivity, reproducibility and specificity for the determination of Ethamsylate in bulk and its tablet dosage forms.
KEYWORDS:
Ethamsylate, HPLC, estimation.
Cite:
P Nagaraju, K Krishnachaithanya, VDN Srinivas, SVN Padma. Reverse Phase High Performance Liquid Chromatographic Method for the Determination of Ethamsylate in Bulk and Tablet Dosage Forms. Asian J. Research Chem. 1(2): Oct.-Dec. 2008; Page 88-90.
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