Journal :   Asian Journal of Research in Chemistry

Volume No. :   1

Issue No. :  2

Year :  2008

Pages :   70-73

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Estimation of Bisoprolol Fumarate in Pharmaceutical Preparations by HPTLC

Address:   RB Kakde*, VH Kotak and DL Kale
Department of Pharmaceutical Sciences, RTM Nagpur University Nagpur-440033 (MS) India.
*Corresponding Author

A novel analytical method for determination of bisoprolol fumarate in tablet dosage form has been developed by HPTLC procedure, using Methanol: Toluene: Ammonia (2:4:0.1, v/v/v) as mobile phase and Merck HPTLC plate precoated 60 F254 silica gel on aluminum sheet as stationary phase. Detection was carried out densitometrically using UV detector at 229 nm. The retention factor (Rf) of bisoprolol fumarate was 0.52±0.02. The linear regression analysis data was used for the regression line in the range of 500-1000 ng/spot for bisoprolol fumarate with correlation coefficient value close to 1.0. The developed method was validated using various validation parameters such as accuracy, precision, specificity, limit of detection, limit of quantitation and ruggedness, which make method as choice for routine quality control analysis for estimation of bisoprolol fumarate in tablet formulation.
Bisoprolol fumarate, HPTLC, Development, Validation.
RB Kakde, VH Kotak, DL Kale. Estimation of Bisoprolol Fumarate in Pharmaceutical Preparations by HPTLC. Asian J. Research Chem. 1(2): Oct.-Dec. 2008; Page 70-73.
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