Journal :   Asian Journal of Research in Chemistry

Volume No. :   2

Issue No. :  1

Year :  2009

Pages :   66-69

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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A Selective High Performance Liquid Chromatographic Method for Estimation of Catechin in Ayurvedic Taila Preparations.



Address:   Nidhi Dubey 1,*, Nitin Dubey 2, Rajendra Mehta3 and Ajay Saluja 3
1School of Pharmacy, Devi Ahilya Vishwavidyalaya, Indore [M P], India.
2College of Pharmacy, IPS Academy, Indore [M P], India
3A. R .College of Pharmacy, Vallabh vidyanagar [Gujarat], India.
* Corresponding Author
DOI No:

ABSTRACT:
A simple, sensitive, rapid and selective high-performance liquid chromatographic (HPLC) method has been developed and validated for the analysis of catechin in marketed Ayurvedic Taila (oil) formulations containing Acacia catechu. Chromatography of methanolic: 0.1% formic acid (7:3, v/v) extracts of these formulations was performed on C18 (5 µm x 25cm x 4.6mm i.d) column using isocratic mobile phase consisting of methanol: acetonitrile: water (40:15:45, v/v/v) containing 1.0% acetic acid at a flow rate of 0.5ml/min and SPD-10 AVP photodiode array (PDA) UV-Visible detector. The analytical marker, catechin, was quantified at 279 nm. The retention time of catechin was about 3.27 min. The linear regression analysis data for the calibration plot showed a good linear relationship with correlation coefficient of 0.9988 in the concentration range of 15 to 90 µ g/ml for catechin with respect to peak area. The limit of detection and limit of quantitation values were found to be 0.5µ g/ml and 1.7µ g/ml respectively. Repeatability of the method was found to be 0.62 RSD. Recovery values from 99.73 to 100.22 % indicate excellent accuracy of the method. The developed HPLC method is accurate, precise, and cost-effective, and it can be successfully applied for the determination of catechin in marketed ayurvedic oil formulations containing Acacia catechu.
KEYWORDS:
Acacia catechu; HPLC; oil; quantitation.
Cite:
Nidhi Dubey, Nitin Dubey, Rajendra Mehta, Ajay Saluja. A Selective High Performance Liquid Chromatographic Method for Estimation of Catechin in Ayurvedic Taila Preparations. Asian J. Research Chem. 2(1): Jan.-March, 2009;Page 66-69.
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