Journal :   Asian Journal of Research in Chemistry

Volume No. :   2

Issue No. :  1

Year :  2009

Pages :   90-92

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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The Estimation of Paclitaxel in Parenterals by RP-HPLC.

Address:   V Kiran Kumar1, N Appala Raju2*, Namratha Rani2, JVLN Seshagiri Rao3 and T Satyanarayana2
1Dept. of Pharma. Chem., Sree Chaitanya Institute of Pharmaceutical Sciences, LMD.Colony, Karimnagar-505527.AP.
2Dept. of Pharma. Chem., Sultan-Ul-Uloom College of Pharmacy, Mount Pleasant, Road 3, Banjara Hills, Hyderabad-500034.
3University College of Pharmaceutical Sciences, Andhra University, Visakhapatnam. AP.
* Corresponding Author

A simple, precise, rapid and accurate reverse phase HPLC method developed for the estimation of Paclitaxel in Parenterals dosage form. A Unison US C18, 250x4.6 mm i.d, 5 m partical size, with mobile phase consisting of Methanol and 0.02 M potassium dihydrogen phosphate in water (pH 2.5 adjusted with o-phosphoric acid) in the ratio of 80:20 v/v was used. The flow rate was 1 ml/min and the effluents were monitored at 225 nm. The retention time was 4.978 min. The detector response was linear in the concentration of 15-180 mcg/ml. The respective linear regression equation being Y= 46411.83x+29.2578. The limit of detection and limit of quantification was 0.03 and 0.09 mcg/ml respectively. The percentage assay of Paclitaxel was 99.09 %. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Paclitaxel in bulk drug and in its pharmaceutical dosage form.
Paclitaxel, RP-HPLC, Estimation, and Parenteral.
V Kiran Kumar, N Appala Raju, Namratha Rani, JVLN Seshagiri Rao, T Satyanarayana. The Estimation of Paclitaxel in Parenterals by RP-HPLC. Asian J. Research Chem. 2(1): Jan.-March, 2009;Page 90-92.
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