Journal :   Asian Journal of Research in Chemistry

Volume No. :   2

Issue No. :  2

Year :  2009

Pages :   131-134

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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HPTLC Method for Quantitative Determination of Gallic Acid in Hydroalcoholic Extract of Dried Flowers of Nymphaea Stellata Willd



Address:   Sachin U Rakesh*, VR Salunkhe, PN Dhabale and KB Burade
Govt. College of Pharmacy, Vidyanagar, Tal. Karad- 415 124, Dist. Satara, M. S. India
*Corresponding Author
DOI No:

ABSTRACT:
A sensitive and reliable high performance thin layer chromatographic method has been developed for quantitation of gallic acid in the dried flowers of Nymphaea stellata willd. The hydroalcoholic extract of N. stellata was chromatographed on silica gel 60 F254 plates with chloroform: ethyl acetate: formic acid, 7.5: 6: 0.5 (v/v/v), as mobile phase. Detection and quantitation were performed by densitrometric scanning at ?= 292 nm, by using deuterium lamp. The accuracy of the method was checked by conducting recovery studies at three different levels, using the standard addition method. The average recovery of gallic acid was found to be 98.33%. The proposed HPTLC method provide a good resolution of gallic acid from other constituents present in hydroalcoholic extract of dried flowers of N. stellata and can be used for quantitation of gallic acid present in extract. The method is rapid, simple and precise.
KEYWORDS:
gallic acid, Nymphaea stellata willd.
Cite:
Sachin U Rakesh, VR Salunkhe, PN Dhabale, KB Burade. HPTLC Method for Quantitative Determination of Gallic Acid in Hydroalcoholic Extract of Dried Flowers of Nymphaea Stellata Willd. Asian J. Research Chem. 2(2): April.-June, 2009 page 131-134.
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