Journal :   Asian Journal of Research in Chemistry

Volume No. :   2

Issue No. :  2

Year :  2009

Pages :   207-209

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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A Simple and Sensitive HPTLC Method for Estimation of Pioglitazone In Bulk and Tablet Dosage Forms



Address:   Meeta A Jiladia*1, SS Pandya2, and Viidyasagar G1
*1Veerayatan Institute of Pharmacy, Jakhania Bhuj-Mandvi Road, Mandvi- 370640 (Gujarat) India
2Babaria Institute of Pharmacy, Vadodara – 391240 (Gujarat) India
*Corresponding Author
DOI No:

ABSTRACT:
A simple and sensitive, HPTLC method has been developed for the quantitative estimation of pioglitazone in its single component tablet formulations (30 mg). Pioglitazone was chromatographed on silica Gel 60 F254 TLC plate using Toluene: methanol: ammonia (7:3:0.1 v/v) as mobile phase. Pioglitazone showed Rf value 0.50±0.03 and scanned at 268 nm using Camag TLC Scanner 3. The method was validated in terms of linearity (200–1200 ng/spot), precision (intra-day variation 0.6 to 3.4%, inter-day variation 0.8 to 3.8%), accuracy (98.7 to 102.5%) and specificity. The limit of detection and limit of quantification for pioglitazone were found to be 5 ng/spot and 20 ng/spot, respectively. The developed method was successfully used for the assay of pioglitazone tablet formulations. The method is simple, sensitive and precise; it can be used for the routine quality control testing of marketed formulations.
KEYWORDS:
Pioglitazone, toluene, methanol, ammonia, HPTLC.
Cite:
Meeta A Jiladia, SS Pandya, Viidyasagar G. A Simple and Sensitive HPTLC Method for Estimation of Pioglitazone In Bulk and Tablet Dosage Forms. Asian J. Research Chem. 2(2): April.-June, 2009 page 207-209
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