Journal :   Asian Journal of Research in Chemistry

Volume No. :   2

Issue No. :  2

Year :  2009

Pages :   115-118

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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A Liquid Chromatography Method for the Simultaneous Determination of Curcumin and Piperine in Food Products Using Diode Array Detection



Address:   Krishna Veni Nagappan*, Meyyanathan S N, Rajinikanth B Raja and Elango Kannan
Department of Pharmaceutical Analysis, J. S. S. College of Pharmacy, Rocklands, Ootacamund-643 001.Tamilnadu, India
*Corresponding Author
DOI No:

ABSTRACT:
A rapid and simple HPLC method has been developed for the simultaneous quantification of curcumin and piperine in food products. Analysis was performed using a C18 column (250 X 4.6 mm) by isocratic elution with 50mM potassium dihydrogen orthophosphate (pH 3.5): Acetonitrile (40:60) and detection at 424 nm and 340 nm using a photodiode array detector for curcumin and piperine respectively. The calibration plot was linear over the range studied (Curcumin: 100 – 3200ng/mL; Piperine: 200 – 7000ng/mL) with a correlation of 0.999. The method was also validated for the precision and recovery. Thus the method is suitable for routine analysis of curcumin and piperine in food products.
KEYWORDS:
Curcumin, Piperine, HPLC, food analysis.
Cite:
Krishna Veni Nagappan, Meyyanathan S N, Rajinikanth B Raja, Elango Kannan. A Liquid Chromatography Method for the Simultaneous Determination of Curcumin and Piperine in Food Products Using Diode Array Detection. Asian J. Research Chem. 2(2): April.-June, 2009 page 115-118.
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