Journal :   Asian Journal of Research in Chemistry

Volume No. :   2

Issue No. :  3

Year :  2009

Pages :   306-309

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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HPTLC estimation of Paracetamol, Diclofenac Sodium and Chlorzoxazone in Tablet dosage form



Address:   Smita J Pawar*, Amol P Kale, Manoj P Amrutkar, Jyotsna J Jagade, Nikhil S Pore and Ashok V Bhosale
PDEA's Seth Govind Raghunath Sable College of Pharmacy, Saswad, Pune-412301
*Corresponding Author
DOI No:

ABSTRACT:
A simple, rapid, reliable and accurate HPTLC method has been developed for the quantitative determination of Paracetamol, Diclofenac Sodium and Chlorzoxazone in tablets. The drugs were extracted from (CIP-ZOX). Various aliquots of this sample solution were spotted automatically by means of Camag (Muttenz; Switzerland) Linomat V sample applicator on Merck HPTLC plates (0.2mm thickness) precoated with silica gel 60 F254 on aluminium sheet as stationary phase prewashed with methanol using Chloroform: Methanol: Ammonia (20:5:0.2v/v/v) as mobile phase. The spots were scanned at ?=254nm using Camag TLC scanner 3. The Rf values of Paracetamol, Diclofenac Sodium and Chlorzoxazone were found to be 0.53, 0.27, and 0.68 respectively. Calibration curves were linear in range of 1000-5000ng per spot. The limit of detection (LOD) and quantitation (LOQ) for Paracetamol, Diclofenac sodium and Chlorzoxazone were found to be 100, 100, 500 and 300, 300, 1500ng per spot respectively. The suitability of this method for quantitative determination of compounds was proved by validation in accordance with requirements of pharmaceutical regulatory standards. The proposed method is valid, simple, sensitive and accurate. Therefore this method can be applied for routine analysis of these drugs in tablet formulation.
KEYWORDS:
, Diclofenac sodium, Chlorzoxazone, HPTLC, Pharmaceutical dosage form.
Cite:
Smita J Pawar, Amol P Kale, Manoj P Amrutkar, Jyotsna J Jagade, Nikhil S Pore, Ashok V Bhosale. HPTLC estimation of Paracetamol, Diclofenac Sodium and Chlorzoxazone in Tablet dosage form. Asian J. Research Chem. 2(3): July-Sept., 2009, page 306-309.
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