Journal :   Asian Journal of Research in Chemistry

Volume No. :   2

Issue No. :  4

Year :  2009

Pages :   565-568

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Purification and Characterization of GroES and GroEL from Genetically Modified Strain E. coli U1/Pus01/Puss1∆Cat



Address:   Alok Kumar Verma and GP Pal
Deptarment of Molecular Biology, IMS BHU Varanasi-221005
*Corresponding Author
DOI No:

ABSTRACT:
In Present study our aim is to purify and characterize GroES and GroEL from genetically modified strain E.coli U1/pUS01/pUSS1?CAT L .GroES was eluted at 0.2M NaCl and GroEL was at 0.3M NaCl. From1.4 litres culture 7.5mg GroEL and 50mg GroES was purified.
KEYWORDS:
Cheperonin, GroEL, GroES, IPTG, Q-Sepharose FF, Sephacryl S-200 HR.
Cite:
Alok Kumar Verma, GP Pal. Purification and Characterization of GroES and GroEL from Genetically Modified Strain E. coli U1/Pus01/Puss1∆Cat. Asian J. Research Chem. 2(4):Oct.-Dec. 2009 page 565-568.
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