Journal :   Asian Journal of Research in Chemistry

Volume No. :   2

Issue No. :  4

Year :  2009

Pages :   574-576

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Development and Validation of HPTLC Method for Simultaneous Estimation of Omeprazole and Ondansetron in Tablet Dosage Form



Address:   Zarna Dedania1*, Ronak Dedania1, G Vidhyasagar1, Bhavna Patel2, Chetan Ramolia3 and Vaishali Karkhanis4
1Department of Pharmaceutical Analysis, Veerayatan Institute of Pharmacy, Kutch
2Ramanbhai Patel College of Pharmacy, Changa, Gujarat India
3CRO, Veeda, Ahmedabad, Gujarat India
4A.R. College of Pharmacy, Vallabh Vidhyanagar
*Corresponding Author
DOI No:

ABSTRACT:
A simple, precise, sensitive, rapid and reproducible HPTLC method for the simultaneous estimation of the omeprazole and ondansetron in tablets was developed and validated. This method involves separation of the components by HPTLC on precoated silica gel G60F254 plate with solvent system of methanol: ethyl acetate: toluene(2:6:2 % v/v) and detection was carried out densitometrically using a UV detector at 280 nm in absorbance mode. This system was found to give compact spots for omeprazole (Rf value of 0.68±0.02) and for ondansetron (Rf value of 0.17±0.02). Linearity was found to be in the range of 1000-3500 ng/spot and 1000-3500 ng/spot for omeprazole and ondansetron respectively. The limit of detection and limit of quantification for omeprazole were 123.42 and 374.01 ng/spot and for ondansetron were 134.63 and 407.97 ng/spot, respectively. The method was found to be beneficial for the routine analysis of combined dosage form.
KEYWORDS:
Simultaneous Estimation; HPTLC; Omeprazole; Ondansetron.
Cite:
Zarna Dedania, Ronak Dedania, G Vidhyasagar, Bhavna Patel, Chetan Ramolia, Vaishali Karkhanis. Development and Validation of HPTLC Method for Simultaneous Estimation of Omeprazole and Ondansetron in Tablet Dosage Form. Asian J. Research Chem. 2(4):Oct.-Dec. 2009 page 574-576.
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