Journal :   Asian Journal of Research in Chemistry

Volume No. :   3

Issue No. :  1

Year :  2010

Pages :   178-182

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Simultaneous Determination of Sodium Benzoate and Potassium Sorbate Preservatives in Foodstuffs Using High-Performance Liquid Chromatography



Address:   Antakli S1, Alahmad A2 and Badingki H1
1Department of Chemistry Faculty of Science University of Aleppo, SYRIA
2Department of Technology of Foodstuff Faculty of Technicality Engineering University of Aleppo, SYRIA
*Corresponding Author
DOI No:

ABSTRACT:
A reversed-phase HPLC analysis without preextraction was carried out for rapid determination of sodium benzoate and potassium sorbate which were added as preservatives to different samples commercially available in Syrian Arab Republic local markets such as tomato sauce, soft drink, beverages and energy drink. Separation and determination of sodium benzoate and potassium sorbate were performed on a 5µm Purospher® STAR RP-18 column (25 cm x 4.6 mm), using caffeine as an internal standard (0.04 mg/ml), with UV detection at 235 nm, The column temperature was 40°C. Mobile phase was (acetate buffer and methanol) with percentage (25:75), At a flow rate 1.2 ml min-1,The injection volume was 20µl. The two elution peaks were completely separated with a good resolution. This method has offered a determination of sodium benzoate and potassium sorbate directly with a high accuracy and authenticity for the results without extraction need.
KEYWORDS:
preservatives, HPLC, sodium benzoate, potassium sorbate.
Cite:
Antakli S, Alahmad A, Badingki H. Simultaneous Determination of Sodium Benzoate and Potassium Sorbate Preservatives in Foodstuffs Using High-Performance Liquid Chromatography. Asian J. Research Chem. 3(1):Jan.-Mar. 2010 page 178-182.
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