Journal :   Asian Journal of Research in Chemistry

Volume No. :   3

Issue No. :  1

Year :  2010

Pages :   188-191

ISSN Print :  0974-4169

ISSN Online :  0974-4150


Registration

Allready Registrered
Click to Login

Development and Validation of Spectrophotometric Method for the Estimation of Lopinavir and Ritonavir in Tablet Dosage Form



Address:   Ilangovan Ponnilavarasan1*, Aiyalu Rajasekaran1, Joghee Gowder Dharuman1, Duraisamy Kalaiyarasi1 and Duraisamy Krishnakumar2
1College of Pharmacy, Kovai Medical Center Research and Educational Trust, Coimbatore, 641048, India
2Shantha Bio-Technics Limited, Hyderabad, 501401, India
*Corresponding Author
DOI No:

ABSTRACT:
A new, simple, rapid and novel spectrophotometric method has been developed for the simultaneous estimation of Lopinavir and Ritonavir using simultaneous equation. The method involved measurement of absorbance at two wavelengths, 259 nm and 240 nm, of Lopinavir and Ritonavir respectively. Beer’s law obeyed in concentration range of 10- 50 µg/ mL and 15 - 55 µg/ mL for Lopinavir and Ritonavir respectively. The proposed method has been applied successfully for the analysis of the drug in pure and in its tablets dosage forms. Result of percentage recovery and placebo interference shows that the method was not affected by the presence of common excipients. The method can be recommended for routine analysis since it is rapid, simple, accurate and also sensitive and specific. This paper describes the development and validation of ultra violet spectroscopic method for simultaneous estimation of Lopinavir and Ritonavir in combined solid dosage form.
KEYWORDS:
Lopinavir, Ritonavir, simultaneous equation method, Beer’s law..
Cite:
Ilangovan Ponnilavarasan1*, Aiyalu Rajasekaran, Joghee Gowder Dharuman, Duraisamy Kalaiyarasi, Duraisamy Krishna kumar. Development and Validation of Spectrophotometric Method for the Estimation of Lopinavir and Ritonavir in Tablet Dosage Form. Asian J. Research Chem. 3(1): Jan.-Mar. 2010; Page 188-191.
[View HTML]      [View PDF]



Visitor's No. :   112360