Journal :   Asian Journal of Research in Chemistry

Volume No. :   3

Issue No. :  2

Year :  2010

Pages :   312-315

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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In Vitro Antioxidant Activity of the Methanolic Extract of Simaruba glauca DC

Address:   Jiby Elias1, Rajesh MG1*, Anish NP1, Deepa P2 and Jayan N1
1Department of Biotechnology, Navajyothi Sree Karunakara Guru Research Centre for Ayurveda and Siddha, Uzhavoor, Kottayam, Kerala, India – 686 634
2Union Christian College, Alwaye, Ernakulam, Kerala, India – 683 102
*Corresponding Author

The present investigation evaluates the in vitro antioxidant potential of the methanolic extract of Simaruba glauca (MESG). The reducing power of the extract increased with the increasing concentration which indicated that the plant has inherent antioxidant potential. MESG at 250 µg/ml showed maximum scavenging activity of DPPH (83.87%) followed by hydroxyl (80.19%), superoxide (76.25%) and nitric oxide (70.43%) radicals. The half inhibition concentration (IC50) of DPPH, hydroxyl, superoxide, and nitric oxide radicals were 21µg/ml, 26µg/ml, 27µg/ml and 32µg/ml respectively against the corresponding reference standards. Phytochemical screening of MESG revealed the presence of alkaloids, flavanoids, sterol/ terpenoids, quinines, anthroquinones, glycoside, reducing sugar, carbohydrate, volatile oil and phenolic compounds. However, coumarins, saponins, tannins and resins were absent in the extract. The total phenolic content of MESG was 24.6mg/g. The findings suggest that MESG possesses potent antioxidant activity and has great importance in nutraceuticals and pharmaceutical preparations.
Simaruba glauca, DPPH, reducing power, phenol.
Jiby Elias, Rajesh MG, Anish NP, Deepa P, Jayan N. In Vitro Antioxidant Activity of the Methanolic Extract of Simaruba glauca DC. Asian J. Research Chem. 3(2): April- June 2010; Page 312-315.
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