Journal :   Asian Journal of Research in Chemistry

Volume No. :   3

Issue No. :  2

Year :  2010

Pages :   329-331

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Derivatized HPTLC Method for Simultaneous Estimation of Glucosamine, Vitamin C and Vitamin E in Tablets



Address:   WD Sam Solomon*, Rahul A Kumar, PR Vijai Anand and R Venkatnarayanan
Dept. of Pharmaceutical analysis, RVS College of Pharmaceutical Sciences, Sulur, Coimbatore- 641 402. Tamilnadu
*Corresponding Author
DOI No:

ABSTRACT:
An accurate, precise and derivatized HPTLC method has been developed for the simultaneous estimation of Glucosamine, Vitamin C and Vitamin E in tablet formulation. In this method standard and sample solutions of Glucosamine, Vitamin C and Vitamin E were applied on pre-coated silica gel 60F254 TLC plate, and developed using ethanol: Acetic acid (9:1 v/v), as mobile phase and derivatized using Iodine vapor. The drugs on the plate were scanned at 500 nm. The dynamic linearity range was 20-100 µg/spot for Glucosamine, 2 - 10 µg/spot for Vitamin C and 0.2 -1 µg/spot for Vitamin E. The method was validated for precision, accuracy and reproducibility.
KEYWORDS:
Simultaneous estimation, HPTLC, Glucosamine, Vitamin C and Vitamin E.
Cite:
WD Sam Solomon, Rahul A Kumar, PR Vijai Anand, R Venkatnarayanan. Derivatized HPTLC Method for Simultaneous Estimation of Glucosamine, Vitamin C and Vitamin E in Tablets. Asian J. Research Chem. 3(2): April- June 2010; Page 329-331.
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