Journal :   Asian Journal of Research in Chemistry

Volume No. :   3

Issue No. :  2

Year :  2010

Pages :   404-406

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Development and Statistical Validation of UV Spectrophotometric Method for Estimation of Griseofulvin in Tablet Dosage Form



Address:   PA Jadhav, CS Raut*, JP Bidada, BB Buwa, PN Dhabale and SC Dhawale
Government College of Pharmacy, Karad, Vidyanagar, Tal. Karad- 415 124, Dist. Satara, Maharashtra, India
*Corresponding Author
DOI No:

ABSTRACT:
A new, simple, rapid and novel reproducible UV-spectrophotometer estimation method has been developed for estimation of griseofulvin in marketed formulation. The proposed method was successfully applied for the estimation of griseofulvin in commercial pharmaceutical preparation with Absorbance maxima at 263.5 nm. A Shimadzu 1700 UV- Visible spectrophotometer with 1cm matched quartz cells and acetone: ethanol: 0.1N HCl (1:1:8) solvents were employed in the method. Developed methods obeyed the Beer’s law in the concentration range of 0.5-3.5 µg/ml and methods were validated statistically. The SD and Percentage recovery of the drug for the proposed method are given in method indicating no interference of the tablet excipients. The results of the tablet analysis were validated with respect to accuracy (recovery), linearity, limit of detection and limit of quantitation were found to be satisfactory.
KEYWORDS:
Absorbance maxima, Area under curve (AUC), Griseofulvin, UV spectrophotometer.
Cite:
PA Jadhav, CS Raut, JP Bidada, BB Buwa, PN Dhabale, SC Dhawale. Development and Statistical Validation of UV Spectrophotometric Method for Estimation of Griseofulvin in Tablet Dosage Form. Asian J. Research Chem. 3(2): April- June 2010; Page 404-406.
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