Journal :   Asian Journal of Research in Chemistry

Volume No. :   3

Issue No. :  3

Year :  2010

Pages :   673-677

ISSN Print :  0974-4169

ISSN Online :  0974-4150


Registration

Allready Registrered
Click to Login

Simultaneous Determination of Ciprofloxacin Hydrochloride and Dexamethasone in Ophthalmic Solution by Reversed Phase High Performance Liquid Chromatography



Address:   Rele R.V.* and Warkar C.B.
D. G. Ruparel College, Matunga, Mumbai 400 016
*Corresponding Author
DOI No:

ABSTRACT:
A gradient reverse phase HPLC method was developed and validated for simultaneous estimation of ciprofloxacin hydrochloride and dexamethasone in pharmaceutical dosage form i.e. ophthalmic solution using Nucleosil C18 (15cm x 4.6mm, 5µm) column with a flow rate of 1.2 ml/minute and detection of 265nm. The mobile phase A consisted of 50 milli molar citric acid and potassium phosphate buffer. The mobile phase B consisted of 100 % v/v acetonitrile. The method was validated for system suitability, linearity, accuracy and precision. The system suitability parameters like resolution, theoretical plates and tailing factor were evaluated. The linear range for ciprofloxacin was 3 µg/ml – 55 µg/ml and for dexamethasone was 1 µg/ml – 16 µg/ml. The method has been successfully used to analyze pharmaceutical dosage form i.e. ophthalmic solution containing ciprofloxacin hydrochloride and dexamethasone with good recoveries.
KEYWORDS:
Ciprofloxacin hydrochloride, Dexamethasone, Pharmaceutical dosage form.
Cite:
Rele R.V.., Warkar C.B.. Simultaneous Determination of Ciprofloxacin Hydrochloride and Dexamethasone in Ophthalmic Solution by Reversed Phase High Performance Liquid Chromatography. Asian J. Research Chem. 3(3): July- Sept. 2010; Page 673-677
[View HTML]      [View PDF]



Visitor's No. :   119855