Journal :   Asian Journal of Research in Chemistry

Volume No. :   3

Issue No. :  3

Year :  2010

Pages :   545-548

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Development and Validation of UV Spectrophotometric Method of Fluoxetine Hydrochloride in Bulk and Pharmaceutical Formulation



Address:   Zahid Zaheer*, Obaid Shaikh, Sucheta Thorat and Rana Z. Ahmed
Y. B. Chavan College of Pharmacy, Dr. Rafiq Zakaria Campus, Aurangabad-431001
*Corresponding Author
DOI No:

ABSTRACT:
A simple, accurate, cost effective and reproducible spectrophotometric method has been developed for the estimation of fluoxetine hydrochloride in bulk and pharmaceutical dosage form. UV spectrophotometric method, which is based on measurement of absorption at maximum wavelength 226nm.The developed method was validated with respect to linearity, accuracy (recovery), precision, specificity, ruggedness and robustness. Beer’s law was obeyed in the concentration range of 5-50µg/ml having line equation y =0.027x+0.030 with correlation coefficient of 0.999. The percentage recovery is 100.01 which reflect that the method is free from interference of the impurities and other additives during the estimation of drug in formulation. The proposed method can be successfully used for analysis of fluoxetine hydrochloride in marketed preparations. Results of the analysis were validated statistically and by recovery study.
KEYWORDS:
UV spectrophotometry, Fluoxetine hydrochloride.
Cite:
Zahid Zaheer, Obaid Shaikh, Sucheta Thorat, Rana Z. Ahmed. Development and Validation of UV Spectrophotometric Method of Fluoxetine Hydrochloride in Bulk and Pharmaceutical Formulation. Asian J. Research Chem. 3(3): July- Sept. 2010; Page 545-548.
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