Journal :   Asian Journal of Research in Chemistry

Volume No. :   3

Issue No. :  3

Year :  2010

Pages :   763-766

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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RP-HPLC Determination of Amlodipine Besylate and Atorvastatin from Tablets Dosage forms



Address:   Ravinder Burugu1* and G. Venkateshwarlu2
1Bioplus Life Sciences Pvt. Ltd.,Whitefield Road, Bangalore-560048
2Department of chemistry, NIZAM College, Osmania University, Hyderabad
*Corresponding Author
DOI No:

ABSTRACT:
A simple, specific, accurate and precise high performance liquid chromatographic method was developed for the determination of Amlodipine and Atorvastatin in Amlodipine besylate and Atorvastatin tablet dosage forms. A Hypersil BDS C18 5µm (250x4.6mm I.D) with column temperature 30°C in isocratic mode, with mobile phase containing Acetonitrile: 10mM Ammonium acetate buffer pH 4.0 (50:50) was used. The flow rate was 1.0mL/min and effluent was monitored at 238nm.The retention time of Amlodipine was 2.5min and for Atorvastatin was 4.7min.The linearity for Amlodipine and Atorvastatin was in the range 0.02mg/mL to 0.12mg/mL.The proposed method is accurate, precise, specific and rapid estimation of Amlodipine and Atorvastatin in Amlodipine besilate and Atorvastatin tablets.
KEYWORDS:
HPLC, Amlodipine besylate (AMD), Atorvastatin (ATVR), Dissolution, Assay, Validation, Isocratic elution.
Cite:
Ravinder Burugu, G. Venkateshwarlu. RP-HPLC Determination of Amlodipine Besylate and Atorvastatin from Tablets Dosage forms. Asian J. Research Chem. 3(3): July- Sept. 2010; Page 763-766.
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