Journal :   Asian Journal of Research in Chemistry

Volume No. :   3

Issue No. :  3

Year :  2010

Pages :   549-551

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Simultaneous Determination of Risperidone and Trihexhyphenidyl Hydrochloride from Bulk and Tablet Dosage Form by RP-HPLC



Address:   V Rajamanickam*, Dharmaraj Santhosam, D. Sridharan, and A. Thenmozhi
Department of Pharmaceutical Analysis, Arulmigu Kalasalingam College of Pharmacy, Anand Nagar, Krishnankoil -626 190. Tamil Nadu
*Corresponding Author
DOI No:

ABSTRACT:
A simple, reverse phase high performance liquid chromatographic (HPLC) method was developed for the determination of Risperidone and Trihexyphenidyl hydrochloride in combined tablet dosage form. A octadecyl silane (ODS) C18 Phenomenex Gemini (250mm X 4.6mm, i.d., 5µ) column , with mobile phase of 0.3% ortho phosphoric acid buffer , acetonitrile and methanol (50:30:20%v/v/v) adjusted to pH3 with triethylamine was used. The flow rate was 1mL/minute and the effluent was monitored at 220nm. The retention times for Risperidone and Trihexyphenidyl hydrochloride were 2.685±0.012 and 5.912±0.030 minutes respectively. The linearity range was found to be 4.8 µg/mL to 19.2 µg/mL for Risperidone and 3.2 µg/mL to 12.8 µg/mL for Trihexyphenidyl hydrochloride. The proposed method was validated as per ICH guidelines.
KEYWORDS:
Risperidone, Trihexyphenidyl hydrochloride, RP-HPLC, Tablets.
Cite:
V Rajamanickam, Dharmaraj Santhosam, D. Sridharan, A. Thenmozhi. Simultaneous Determination of Risperidone and Trihexhyphenidyl Hydrochloride from Bulk and Tablet Dosage Form by RP-HPLC. Asian J. Research Chem. 3(3): July- Sept. 2010; Page 549-551.
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