Journal :   Asian Journal of Research in Chemistry

Volume No. :   3

Issue No. :  3

Year :  2010

Pages :   805-808

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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A Validated Reverse Phase HPLC method for the Simultaneous Estimation of Ritonavir and Lopinavir in Pharmaceutical Dosage Forms



Address:   Varun Dasari1*, Bahlul Z. Awen2, Babu Rao Chandu2, Mukkanti Khagga3and Nagaraju Pappula1
1Hindu College of Pharmacy, Guntur-522002, Andhrapradesh, India
2College of Pharmacy, Al-Jabal Al-Gharbi University, Al- Zawia, Libya
3Centre for Pharmacy, JNT University, Hyderabad, Andhra Pradesh, India
*Corresponding Author
DOI No:

ABSTRACT:
A simple, sensitive and precise reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of Ritonavir and Lopinavir in pharmaceutical dosage forms. The mobile phase consisted of Acetonitrile: buffer (0.05M Potassium dihydrogen orthophosphate, PH – 3) in the ratio of 50:50 v/v delivered at a flow rate of 2.0 ml / min and wavelength of detection at 215nm. The retention times of Ritonavir and Lopinavir were 10.253 min and 12.490 min respectively. The developed method was validated according to ICH guidelines. The proposed method can be used for determination of these drugs in combined dosage forms.
KEYWORDS:
Ritonavir, Lopinavir, RP-HPLC.
Cite:
Varun Dasari, Bahlul Z. Awen, Babu Rao Chandu, Mukkanti Khagga, Nagaraju Pappula. A Validated Reverse Phase HPLC method for the Simultaneous Estimation of Ritonavir and Lopinavir in Pharmaceutical Dosage Forms. Asian J. Research Chem. 3(3): July- Sept. 2010; Page 805-808.
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