Journal :   Asian Journal of Research in Chemistry

Volume No. :   3

Issue No. :  4

Year :  2010

Pages :   865-868

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Analytical Method Development and Validation of Cefixime and Dicloxacillin Tablet by RP-HPLC



Address:   Kathiravan S.*, Anbarasi B. and Mathankumar S.
Department of Pharmaceutical Analysis, J.K.K.Munirajah Medical Research Foundation College of Pharmacy, Komarapalayam, Namakkal-Dt, Tamilnadu, India-638183
*Corresponding Author
DOI No:

ABSTRACT:
A simple and sensitive high-performance liquid chromatography method with ultraviolet (UV) detection has been developed and validated procedure was developed for simultaneous determination of Cefixime and Dicloxacillin in tablet dosage form by reverse phase C18 column (water symmetry C18 5ยต, 250mm x 4.6mm). The mobile phase used as combination of Acetonitrile: Potassium dihydrogen Phosphate in the ratio of 60:40 (pH adjusted to 6.0 with potassium hydroxide). The detection of the combined dosage form was carried out at 220 nm and flow rate of 1.0 ml/min. The retention time for Cefixime and Dicloxacillin was found to be 2.027 and 3.249 min respectively, and recoveries from combined dosage form were between 98% to 102%. Linearity obtained in the concentration ranges from 60, 80, 100, 120, 140% Cefixime and Dicloxacillin with correlation coefficient of 0.9991 and 0.9996 respectively. The method herein described was successfully applied for the evaluation of Cefixime and Dicloxacillin combined dosage form.
KEYWORDS:
Cefixime, Dicloxacillin and HPLC.
Cite:
Kathiravan S., Anbarasi B., Mathankumar S.. Analytical Method Development and Validation of Cefixime and Dicloxacillin Tablet by RP-HPLC. Asian J. Research Chem. 3(4): Oct. - Dec. 2010; Page 865-868.
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