Journal :   Asian Journal of Research in Chemistry

Volume No. :   3

Issue No. :  4

Year :  2010

Pages :   922-927

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Simultaneous Determination of Levofloxacin and Ornidazole by Ratiospectra Derivative Spectrophotometry and High Performance Liquid Chromatography



Address:   Parag R. Patel1, Vandana B. Patel2* and Mayank Bapna2
1Pharmaceutical Quality Assurance Laboratory, Pharmacy Department, Faculty of Technology and Engineering, The Maharaja Sayajirao University of Baroda, Vadodara 390 001, Gujarat (India)
2 Baroda College of Pharmacy, P.O. Limda, Ta. Waghodia, Dist. Vadodara, Pin 391 760 Gujarat (India)
*Corresponding Author
DOI No:

ABSTRACT:
Rapid, precise, accurate and specific ratiospectra derivative spectrophotometry and high-performance liquid chromatography procedures were described for the simultaneous determination of levofloxacin and ornidazole in their combined dosage forms. For the first method of ratiospectra derivative spectrophotometry the signals were measured at 302 nm for ornidazole and 292.8 nm for levofloxacin as a mixture. The second method is based on high performance liquid chromatography (HPLC) on Princeton RP-C18 column (5µ, 150x4.6mm) using 0.1% v/v triethylamine-acetonitrile (80:20, v/v) (pH 6.0). Detection was carried out using a UV detector at 295 nm. The retention time for levofloxacin and ornidazole were found to be 2.8 min and 4.4 min respectively. Both the methods showed good linearity in the range of 1 to 20µg/mL and 5 to 5000ng/ml for ratiospectra derivative spectroscopy and HPLC respectively. Pharmaceutical formulations containing both drugs were successfully assayed using the developed methods.
KEYWORDS:
Levofloxacin, Ornidazole, Ratiospectra derivative spectrophotometry, High-performance liquid chromatography, Pharmaceutical formulations.
Cite:
Parag R. Patel, Vandana B. Patel, Mayank Bapna. Simultaneous Determination of Levofloxacin and Ornidazole by Ratiospectra Derivative Spectrophotometry and High Performance Liquid Chromatography. Asian J. Research Chem. 3(4): Oct. - Dec. 2010; Page 922-927.
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