Journal :   Asian Journal of Research in Chemistry

Volume No. :   3

Issue No. :  4

Year :  2010

Pages :   928-931

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Development and Validation of Spectrophotometric Methods for Estimation of Granisetron Hydrochloride in Pure and it’s Pharmaceutical Dosage Forms



Address:   Balap Aishwarya R.1*, Prasad Deepshikha V.1, Khidse Anuja S.1, Jadhav Shailaja B.1, Joshi S.V.2 and Chaudhari Praveen D.1
1PES’s Modern College of Pharmacy, Yamunanagar, Nigdi, Pune-411 044 2PES’s Modern College of Pharmacy [For Ladies], Borhadewadi, Dehu-Alandi Road- Moshi, Pune-412105 *Corresponding Author
DOI No:

ABSTRACT:
Three simple, precise and economical UV methods have been developed for the determination of Granisetron hydrochloride in pure and it’s pharmaceutical dosage forms. Granisetron hydrochloride has the absorbance maxima at 302 nm (Method A) in phosphate buffer (pH 7.0). The quantitative determination of the drug was carried out using the first order derivative, values measured at 241.5 nm (Method B) and Area under Curve (AUC) for analysis in the wavelength range of 299-305 nm (Method C). Calibration graph constructed at 302 nm was linear in concentration range of 2-10 µg/ml with correlation coefficient 0.9994. The proposed methods were successfully applied for the determination of Granisetron hydrochloride in commercial tablet preparation. Results of the analysis were validated statistically and by recovery studies and were found to be satisfactory.
KEYWORDS:
Granisetron hydrochloride, Absorbance maxima, Derivative spectrophotometry, Area under curve.
Cite:
Balap Aishwarya R., Prasad Deepshikha V., Khidse Anuja S., Jadhav Shailaja B., Joshi S.V., Chaudhari Praveen D.. Development and Validation of Spectrophotometric Methods for Estimation of Granisetron Hydrochloride in Pure and it’s Pharmaceutical Dosage Forms. Asian J. Research Chem. 3(4): Oct. - Dec. 2010; Page 928-931.
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