Journal :   Asian Journal of Research in Chemistry

Volume No. :   3

Issue No. :  4

Year :  2010

Pages :   1062-1064

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Simultaneous Estimation of Nitazoxanide and Ofloxacin by RP-HPLC Method



Address:   Jayalakshmi B.*, Ramesh J., Navin Kumar M. and Vijayamirtharaj R.
Department of Pharmaceutical Analysis, JKK Munirajah Medical Research Foundation College of Pharmacy, Komarapalayam. 638 183. Namakkal (DT), Tamilnadu
*Corresponding Author
DOI No:

ABSTRACT:
The present paper deals with the development of RP-HPLC method for the determination of Nitazoxanide and Ofloxacin in bulk and in formulation using UV detector. Selected mobile phase was a combination of 0.005M tetra butyl ammonium hydrogen sulphate and acetonitrile in the ratio of 45:55and the wavelength selected was 240nm. The flow rate 1.0ml / min, and the injection volume was 20┬Ál. The sepration was performed at ambient temperature. Retention time of Nitazoxanide and Ofloxacin was found to be 6.051and 2.106. Linearity of the method was found to be 400 to 600 mcg/ml and 160 to 240 mcg/ml for Nitazoxanide and Ofloxacin respectively. Accuracy of the method was determined through recovery studies by adding known quantities of standard drug to the pre analyzed test solution and was found to be 99.08-99.81% and 100.18-100.65% for Nitazoxanide and Ofloxacin respectively. This method was validated according to ICH guidelines.
KEYWORDS:
Nitazoxanide, Ofloxacin and RP-HPLC.
Cite:
Jayalakshmi B., Ramesh J., Navin Kumar M., Vijayamirtharaj R.. Simultaneous Estimation of Nitazoxanide and Ofloxacin by RP-HPLC Method. Asian J. Research Chem. 3(4): Oct. - Dec. 2010; Page 1062-1064.
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