Journal :   Asian Journal of Research in Chemistry

Volume No. :   4

Issue No. :  1

Year :  2011

Pages :   24-27

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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RP-HPLC Determination of Amitriptyline Hydrochloride in Tablet Formulations and Urine


Rabie S. Farag, Ashraf M. Ahmed

ashraf_m1974@yahoo.com , chemistashraf2010@hotmail.com

Address:   Rabie S. Farag1 and Ashraf M. Ahmed2*
1Prof. of Inorganic and Analytical Chemistry, Faculty of Science, Al-Azhar University, Cairo, Egypt
2Forensic Chemist, Medico-Legal Organization, Ministry of Justice, Cairo, Egypt
*Corresponding Author
DOI No:

ABSTRACT:
A new, rapid and sensitive reverse phase HPLC method was developed and validated for the determination of amitriptyline hydrochloride in tablet formulations and urine. The mobile phase used acetonitrile and water, (50 % v/v) adjust pH to 5 using phosphoric acid. The separation was achieved on C18 reversed-phase column (250 mm x 4 mm i.d.). The flow rate was 0.6 ml/min and UV detection at 254nm. The retention time for amitriptyline hydrochloride was 7.3 min. The calibration curve was linear in the range 0.5-3 µg/mL. The mean recovery for amitriptyline hydrochloride is 100.025 %. The assay was precise within day and between days. The method provided excellent sensitivity, recovery, accuracy and reproducibility in therapeutic or toxic concentrations. Common excipients do not interfere.
KEYWORDS:
Amitriptyline hydrochloride, Reverse phase HPLC and C18 reversed-phase column.
Cite:
Rabie S. Farag, Ashraf M. Ahmed. RP-HPLC Determination of Amitriptyline Hydrochloride in Tablet Formulations and Urine. Asian J. Research Chem. 4(1): January 2011; Page 24-27.
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