Journal :   Asian Journal of Research in Chemistry

Volume No. :   4

Issue No. :  1

Year :  2011

Pages :   24-27

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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RP-HPLC Determination of Amitriptyline Hydrochloride in Tablet Formulations and Urine

Address:   Rabie S. Farag1 and Ashraf M. Ahmed2*
1Prof. of Inorganic and Analytical Chemistry, Faculty of Science, Al-Azhar University, Cairo, Egypt
2Forensic Chemist, Medico-Legal Organization, Ministry of Justice, Cairo, Egypt
*Corresponding Author

A new, rapid and sensitive reverse phase HPLC method was developed and validated for the determination of amitriptyline hydrochloride in tablet formulations and urine. The mobile phase used acetonitrile and water, (50 % v/v) adjust pH to 5 using phosphoric acid. The separation was achieved on C18 reversed-phase column (250 mm x 4 mm i.d.). The flow rate was 0.6 ml/min and UV detection at 254nm. The retention time for amitriptyline hydrochloride was 7.3 min. The calibration curve was linear in the range 0.5-3 µg/mL. The mean recovery for amitriptyline hydrochloride is 100.025 %. The assay was precise within day and between days. The method provided excellent sensitivity, recovery, accuracy and reproducibility in therapeutic or toxic concentrations. Common excipients do not interfere.
Amitriptyline hydrochloride, Reverse phase HPLC and C18 reversed-phase column.
Rabie S. Farag, Ashraf M. Ahmed. RP-HPLC Determination of Amitriptyline Hydrochloride in Tablet Formulations and Urine. Asian J. Research Chem. 4(1): January 2011; Page 24-27.
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