Journal :   Asian Journal of Research in Chemistry

Volume No. :   4

Issue No. :  1

Year :  2011

Pages :   140-142

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Development and Validation of High Performance Liquid Chromatographic Method for Estimation of Deflazacort in Pharmaceutical Formulation.


D.D. Chougule, N.S. Naikwade

dr_naikwade@rediffmail.com

Address:   D.D. Chougule* and N.S. Naikwade
Appasaheb Birnale College of Pharmacy, Sangli. South-Shivaji Nagar, Sangli 416416 (MS)
*Corresponding Author
DOI No:

ABSTRACT:
A simple, selective, rapid and precise reverse phase HPLC method has been developed for the estimation of Deflazacort in pharmaceutical dosage form. A Hypersil BDS C18 (250 mm X 4.6mm) column was used for separation. The mobile phase was ammonium acetate buffer solution: acetonitrile (50:50 v/v). Flow rate 1.0ml/min with detection at 254 nm. The retention time of Deflazacort was 6.03min. Linearity obtained in the concentration range of 20-100µg/ml with correlation coefficient of 0.998.The result of the analysis were validated statistically and recovery studies confirmed by the accuracy of the proposed method. The proposed RP-HPLC method for the estimation of Deflazacort in dosage form is accurate, precise, linear, robust, simple, rapid and selective.
KEYWORDS:
RP-HPLC, Deflazacort.
Cite:
D.D. Chougule, N.S. Naikwade. Development and Validation of High Performance Liquid Chromatographic Method for Estimation of Deflazacort in Pharmaceutical Formulation. Asian J. Research Chem. 4(1): January 2011; Page 140-142.
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