Journal :   Asian Journal of Research in Chemistry

Volume No. :   4

Issue No. :  10

Year :  2011

Pages :   1505-1507

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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HPTLC Method for Estimation of Cavinton in Bulk Dosage Form

Address:   Raksha L. Mhetre*, Vishal B. Hol and Amrita N. Bajaj
C.U.Shah College of Pharmacy, Santacruz, Mumbai, Maharashtra, India.
*Corresponding Author

A simple, precise, sensitive, rapid and reproducible HPTLC method has been developed and validated for the estimation of Cavinton in bulk doasage form. Cavinton was chromatographed on precoated silica gel 60F 254 TLC plate (0.2 mm thickness) with solvent system of Petroleum ether: Ethyl acetate: Glacial acetic acid (2.5:2.5:0.0.5). The Rf value of Cavinton was 0.411 and scanned at 268 nm in absorbance mode, dentiometrically using a camag TLC scanner 3. The applicability of the method for Cavinton determination was verified by the determination of these compounds in marketed tablets dosage form. The results of analysis were validated statistically and by recovery studies (99.60-100.05%). The recovery and RSD values were within limits as given in ICH guidelines, method development indicates that the suitability of proposed method for the routine determination of these compounds in tablets dosage forms. The validation parameters; Linearity was found to be in the range of 2000-7000 ng /spot for Cavinton. with significantly high value of correlation coefficient (r2 = 0.998). The method was validated for precision, robustness and recovery. The limit of detection was found to be 200ng/spot and limit of quantitation was found to be 1000ng/spot. The proposed method can be successfully used to determine the drug content in marketed formulation.
Cavinton, HPTLC, Validation.
Raksha L. Mhetre, Vishal B. Hol, Amrita N. Bajaj. HPTLC Method for Estimation of Cavinton in Bulk Dosage Form. Asian J. Research Chem. 4(10): Oct., 2011; Page 1505-1507.
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