Journal :   Asian Journal of Research in Chemistry

Volume No. :   4

Issue No. :  10

Year :  2011

Pages :   1649-1651

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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The Estimation of Etravirine in Tablet dosage form by RP-HPLC



Address:   L. Satyanarayana*, S.V. Naidu, M. Narasimha Rao and D. Priyadarshini
Department of Pharmaceutical Chemistry, Omega College of Pharmacy, Edulabad, Ghatkesar, Ranga Reddy, Hyderabad-500 034.
Centre for Biotechnology, Department of Chemical Engineering, College of Engineering (A), Andhra University, Vishakapatnam - 530003
*Corresponding Author
DOI No:

ABSTRACT:
A simple, precise, rapid and accurate reverse phase HPLC method was developed for the estimation of Etravirine in tablet dosage form. A RP Inertsil ODS-3V C-18, 250x4.6 mm, 5µm partical size, with mobile phase consisting of 0.03M Potassium Dihydrogen orthophosphate in water pH-3.2 with orthophosphoric acid and Acetonitrile in the ratio of 30:70 v/v was used. The flow rate was 1.0 ml/min and the effluents were monitored at 309 nm. The retention time was 9.111 min. The detector response was linear in the concentration of 80-240µg/ml. The respective linear regression equation being Y= 58030X + 86395.9 the limit of detection and limit of quantification was 0.1µg and 0.3µg/ml respectively. The percentage assay of Etravirine was 98.46%. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Etravirine in bulk drug and in its pharmaceutical dosage form.
KEYWORDS:
Etravirine, RP-HPLC, Estimation, and Tablets.
Cite:
L. Satyanarayana, S.V. Naidu, M. Narasimha Rao, D. Priyadarshini. The Estimation of Etravirine in Tablet dosage form by RP-HPLC. Asian J. Research Chem. 4(10): Oct., 2011; Page 1649-1651.
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