Journal :   Asian Journal of Research in Chemistry

Volume No. :   4

Issue No. :  2

Year :  2011

Pages :   227-230

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Simultaneous RP-HPLC Estimation of Ciprofloxacin Hydrochloride and Ornidazole in Tablet Dosage Form



Address:   I. Carolin Nimila*, P. Balan, R. Sathiya Sundar, J. Ashok Kumar and S. Rajasekar
Dept. of Pharmaceutical Analysis, Faculty of Pharmacy, PRIST University, Thanjavur -614904, Tamilnadu, India
*Corresponding Author
DOI No:

ABSTRACT:
A high performance liquid chromatographic method has been proposed for the simultaneous determination of ciprofloxacin hydrochloride and ornidazole in pure and dosage form. The HPLC instrument of Agilent technologies (Agilent 1120 LC Germany) model was used where the drug was chromatographed on a C-18 Zorabax column (4.6mm x 250mm) using a mixture of acetonitrile and water (Millipore Q, pH 3.0 adjusted with O-phosphoric acid) in the ratio of (45:55v/v) as the mobile phase at a flow rate of 1ml/min and the detection was done at 299nm (isobestic point). The retention time for ciprofloxacin HCL and ornidazole were 1.96 and 4.33 min respectively. The calibration curves were found to be linear in the range of 12 to 20 µg/ml with a correlation coefficient of 0.9998 and 0.9997 respectively. The results of analysis have been validated statistically and by recovery studies. The percentage recovery was obtained for ciprofloxacin HCL and ornidazole of 99.86 to 100.14% and 100.40 to100.53% respectively. The simplicity and accuracy of the proposed method ensures its use in routine quality control analysis of pharmaceutical formulations.
KEYWORDS:
ciprofloxacin, ornidazole, RP-HPLC, Analytical method development, Validation.
Cite:
I. Carolin Nimila, P. Balan, R. Sathiya Sundar, J. Ashok Kumar, S. Rajasekar. Simultaneous RP-HPLC Estimation of Ciprofloxacin Hydrochloride and Ornidazole in Tablet Dosage Form. Asian J. Research Chem. 4(2): February 2011; Page 227-230.
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