Journal :   Asian Journal of Research in Chemistry

Volume No. :   4

Issue No. :  4

Year :  2011

Pages :   640-646

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Spectrophotometric and Spectrofluorimetric Methods for Simultaneous Estimation of Amlodipine and Metformin in Bulk and Synthetic Mixture.



Address:   Tejash H. Serasiya1*, Satish Y. Gabhe2 and Nurudin P. Jivani3
1Department of Pharmaceutical Sciences, Jaipur National University, Jaipur - 302 025, India.
2C. U. Shah College of Pharmacy, S.N.D.T. Women’s University, Mumbai - 400 049, India.
3Smt. R. B.Patel Mahila Pharmacy College, Atkot - 360 040, India.
*Corresponding Author
DOI No:

ABSTRACT:
Purpose: the purpose of this article is to develop two simple, rapid, accurate and economical spectrophotometric methods and a sensitive spectrofluorimetric method for the simultaneous estimation of amlodipine (AD) and metformin (MT) in bulk and synthetic mixture. Method: The Spectrophotometric determination involved simultaneous equations method that measured the absorbances of both the drugs at 238 nm (?max of metformin) and at 361 nm (?max of amlodipine) and Q- absorbance ratio method that measured the absorbance of both the drugs at 238 nm (?max of metformin) and at isoabsorptive point (256 nm). A spectrofluorimetric determination is based on the reaction between the drugs and 1-dimethylaminonaphthalene-5-sulphonyl chloride (Dansyl chloride) in presence of 1 M sodium carbonate (pH 10) to yield a highly fluorescent derivative that is measured at 450 nm and 520 nm after excitation at 345 nm for AD and MT, respectively. The different experimental parameters affecting the development and stability of the reaction product were carefully studied and optimized. Results and discussions: Both spectrophotometry methods showed linearity over the range of 5-90 µg ml-1 for AD and 1-10 µg ml-1 for MT. The fluorescence intensity were linear over the range of 0.1-3.5 µg ml-1 for AD and 0.05-1.3 µg ml-1 for MT. Results of analyses were validated statistically and through recovery studies.
KEYWORDS:
spectrophotometric; simultaneous equation method; Q absorbance ration; spectrofluorimetric.
Cite:
Tejash H. Serasiya, Satish Y. Gabhe, Nurudin P. Jivani. Spectrophotometric and Spectrofluorimetric Methods for Simultaneous Estimation of Amlodipine and Metformin in Bulk and Synthetic Mixture. Asian J. Research Chem. 4(4): April, 2011; Page 640-646.
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