Journal :   Asian Journal of Research in Chemistry

Volume No. :   4

Issue No. :  5

Year :  2011

Pages :   737-740

ISSN Print :  0974-4169

ISSN Online :  0974-4150


Registration

Allready Registrered
Click to Login

Simultaneous Determination of Saccharin and Aspartame in Drinks by UV- Spectrophotometry



Address:   Mohd. Idris1*, S.N. Rasool1, K.M. Varshney1, S.K. Shukla2 and T.R. Baggi3
1Central Forensic Science Laboratory, Directorate of Forensic Science, Ministry of Home Affairs, Govt. of India, Ramanthapur, Hyderabad, India.
2Central Forensic Science Laboratory, Directorate of Forensic Science, Ministry of Home Affairs, Govt. of India, Chandigarh, India.
3Forensic Science Unit, Department of Chemistry, Osmania University, Hyderabad, India.
*Corresponding Author
DOI No:

ABSTRACT:
A simple and relatively less expensive UV spectrophotometric method was developed for simultaneous determination of saccharin and aspartame in soft drinks by multicomponent analysis method. Wavelengths of 235 nm and 257 nm were selected for determination of saccharin and aspartame respectively. The limit of detection for saccharin and aspartame was found to be 0.0015 g L-1 and 0.04 g L-1 respectively. The method obeys beers law within the concentration range of 0.002 g L-1 to 0.04 g L-1 and 0.06 g L-1 to 0.28 g L-1 for saccharin and aspartame respectively. This method was found to have the coefficient correlation (r2) value of 0.9997 and 0.999 for saccharin and aspartame respectively. The method was validated for accuracy, precision and repeatability. The proposed method was found to be simple, accurate, reproducible, sensitive, free form interferences, inexpensive and having good applicability to the routine analysis of samples of foods and beverages.
KEYWORDS:
Saccharin, Aspartame, Blend, Multiple-component, Drinks.
Cite:
Mohd. Idris, S.N. Rasool, K.M. Varshney, S.K. Shukla, T.R. Baggi. Simultaneous Determination of Saccharin and Aspartame in Drinks by UV- Spectrophotometry. Asian J. Research Chem. 4(5): May, 2011; Page 737-740.
[View HTML]      [View PDF]



Visitor's No. :   126925