Journal :   Asian Journal of Research in Chemistry

Volume No. :   4

Issue No. :  5

Year :  2011

Pages :   746-749

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Simultaneous Spectrophotometric Estimation of Salbutamol Sulphate and Ambroxol Hydrochloride by Using Various Solvent in Bulk as well as in Tablet Formulation.



Address:   Sohan Chitlange*, Sagar Pandkar, Mukund Tawar and Sagar Wankhede
Padm. Dr. D.Y. Patil Institute of Pharmaceutical sciences and Research, Sant Tukaram Nagar, Pimpri, Pune-411018, Maharashtra, India.
*Corresponding Author
DOI No:

ABSTRACT:
Two UV spectrophotometric methods have been developed for simultaneous estimation of salbutamol sulphate (SAL) and ambroxol hydrochloride (AMB) in combination using distilled water, hydrochloric Acid (0.1N) and phosphate buffer (pH 6.8) as solvent in bulk as well as in tablet formulation. Method I employs formation and solving of simultaneous equation using 224.5 nm (?max for Salbutamol) and 244.5 nm (?max for Ambroxol) as two analytical wavelengths for both drugs in distilled water, hydrochloric acid (0.1N) and phosphate buffer (pH 6.8). Method II involves area under curve method, wavelength range selected are 229.5 nm-219.5 nm for salbutamol and 249.5nm-239.5nm for ambroxol respectively. Beer’s law was obeyed in the concentration range of 4-24 µg/ml and 10 – 60 µg/ml for salbutamol and ambroxol respectively. The methods were validated statistically for accuracy, precision and recovery studies which show no significant difference between the results obtained by the two methods for analysis of salbutamol and ambroxol. The wide linearity range, sensitivity, accuracy, and simple procedure imply that the proposed technique demonstrated to be appropriate for determining assay of both the drug component simultaneously and for dissolution study to find out invitro release rate in tablet formulation.
KEYWORDS:
Salbutamol sulphate, ambroxol hydrochloride, simultaneous equation method, area under curve method.
Cite:
Sohan Chitlange, Sagar Pandkar, Mukund Tawar, Sagar Wankhede. Simultaneous Spectrophotometric Estimation of Salbutamol Sulphate and Ambroxol Hydrochloride by Using Various Solvent in Bulk as well as in Tablet Formulation. Asian J. Research Chem. 4(5): May, 2011; Page 746-749.
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