Journal :   Asian Journal of Research in Chemistry

Volume No. :   4

Issue No. :  5

Year :  2011

Pages :   770-774

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Analytical Methods Validation for Estimation of Squalene from the Rajgeera Seed (Amaranth paniculata)



Address:   Omprakash H. Nautiyal*1, Krishna K. Tiwari2 and Rekha S. Singhal3
1ICT and Professor of Organic Chemistry & Natural Products Chemistry, Lovely Professional University, Chaheru, Jalandhar GT Road, Phagwara, Punjab-144402, India.
2Visiting Professor (JUET), formerly Professor at Institute of Chemical Technology (UDCT), N.M. Parekh Road, Matunga (E), Mumbai-400019, India.
3Professor of Foods, Institute of Chemical Technology (UDCT), Department of Foods and Fermentation, N. M. Parekh Road, Matunga (E), Mumbai-400019, India.
*Corresponding Author
DOI No:

ABSTRACT:
Rajgeera seed (Amaranth paniculata) is now researched as rich source of squalene, earlier source reported was shark liver oil. Squalene was obtained by extraction with SFE and conventional techniques. The Squalene was estimated in the extracted oil using various techniques like UV-Visible spectrophotometer, Iodometric titration, Fourier transform Infra-red spectrophotometer and Gas chromatography. All the analytical techniques were validated by establishing calibrated graph. Out of all these gas chromatography was found to be fast, accurate and reproducible for the estimation of squalene.
KEYWORDS:
Amaranth paniculata, analysis, squalene, quality, chromatography, sophisticated analytics.
Cite:
Omprakash H. Nautiyal, Krishna K. Tiwari, Rekha S. Singhal. Analytical Methods Validation for Estimation of Squalene from the Rajgeera Seed (Amaranth paniculata). Asian J. Research Chem. 4(5): May, 2011; Page 770-774.
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