Journal :   Asian Journal of Research in Chemistry

Volume No. :   4

Issue No. :  5

Year :  2011

Pages :   786-790

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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UV Spectroscopic and Stability-Indicating TLC- Densitometric Method for Simultaneous Estimation of Salbutamol sulphate and Prednisolone in Pharmaceutical Dosage Form



Address:   Sohan S. Chitlange*, Kaushalendra K. Chaturvedi, Sneha R. Tawargeri and Sagar B. Wankhede
Padmashree Dr. D.Y. Patil Institute of Pharmaceutical sciences and Research Sant Tukaram Nagar, Pimpri, Pune-411018, Maharashtra, India.
*Corresponding Author
DOI No:

ABSTRACT:
Rapid, accurate and sensitive method for simultaneous determination of salbutamol sulphate (SAL) and prednisolone (PRE) in pharmaceutical dosage form was developed in the present work. The first developed method wasArea under curve method, wavelength selected is232-222nm for salbutamol and 249-239nm for prednisolone respectively. Linearity was observed in concentration range of 6-20µg/ml for salbutamol as well as for prednisolone. Second developed method was high performance thin layer chromatography (HPTLC). The mobile phase selected was Ethyl acetate: Chloroform: methanol (6.0: 4.0:1.0 v/v/v) with UV detection at 230 nm. Retention factor for SAL and PRE were found to be 0.17 ± 0.02 and 0.52 ± 0.03 respectively. For HPTLC method, linearity was observed in the concentration range of 1-6µg/band for salbutamol and 2-6µg/band for prednisolone and drugs was subjected to oxidation, acid hydrolysis, base hydrolysis and sun light to apply stress condition for degradation studies. Results of analysis were validated statistically and by recovery studies.
KEYWORDS:
Salbutamol sulphate, Prednisolone, Area under curve method, TLC-densitometryand degradation studies.
Cite:
Sohan S. Chitlange, Kaushalendra K. Chaturvedi, Sneha R. Tawargeri, Sagar B. Wankhede. UV Spectroscopic and Stability-Indicating TLC- Densitometric Method for Simultaneous Estimation of Salbutamol sulphate and Prednisolone in Pharmaceutical Dosage Form. Asian J. Research Chem. 4(5): May, 2011; Page 786-790.
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