Journal :   Asian Journal of Research in Chemistry

Volume No. :   4

Issue No. :  6

Year :  2011

Pages :   928-931

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Validated HPTLC Method for Simultaneous Quantitation of Esomeprazole and Domperidone in Combined Capsule Dosage Form



Address:   P. Asha*, I. Ponnilavarasan, Ch. Sunil Narendara Kumar and J. Dharuman
Dept. of Pharmaceutical Analysis, K.M.C.H College of Pharmacy, Kalapatti Road,Coimbatore-641048. Tamilnadu.
*Corresponding Author
DOI No:

ABSTRACT:
A simple, precise, rapid, selective and economical high performance thin layer chromatographic (HPTLC) method has been established for simultaneous analysis of Esomeprazole and Domperidone in capsule dosage forms. The chromatographic separation was performed on precoated silica gel 60 GF254 plates with Acetonitrile: Chloroform: Ammonia (12:7. 5:0. 5)(v/v/v)as a mobile phase. The drugs on the plate were scanned at 222 nm. The drugs were satisfactorily resolved with Rf 0. 31 for domperidone and 0. 48 for esomeprazole. The method was validated for linearity, accuracy, precision, and specificity. The calibration plot was linear between 200-1000 ng/spot for esomeprazole and domperidone. The limit of detection and quantification for esomeprazole was 85. 07 and 255. 21ng/spot and domperidone was 79. 07 and 237. 23 ng/spot. The percent recovery study was done by standard addition method found in the range of 98. 7% and 99. 1% respectively. The proposed HPTLC method is economic, sensitive, and less time consuming than other chromatographic procedures.
KEYWORDS:
Denisitometry, Quantification, Esomeprazole, Domperidone.
Cite:
P. Asha, I. Ponnilavarasan, Ch. Sunil Narendara Kumar, J. Dharuman. Validated HPTLC Method for Simultaneous Quantitation of Esomeprazole and Domperidone in Combined Capsule Dosage Form. Asian J. Research Chem. 4(6): June, 2011; Page 928-931.
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