Journal :   Asian Journal of Research in Chemistry

Volume No. :   4

Issue No. :  6

Year :  2011

Pages :   968-970

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Spectrophotometric Determination of Mesalamine by Diazotization Coupling Reaction in Solid Dosage Forms



Address:   Venugopal Darak*, Arvind B. Karadi, Md. Arshad and S. Appal Raju
Department of Pharmaceutical Analysis, HKES’s College of pharmacy, Sedam road, Gulbarga, Karnataka-585105, India.
*Corresponding Author
DOI No:

ABSTRACT:
Two simple, sensitive spectrophotometric methods (Method A and B) are developed based on the diazotization of Mesalamine with nitrous acid (NaNo2/ HCl) at cold temperature followed by its coupling in-situ with Chromotropic acid (Method A) to yield cherry red coloured chromogen, phloroglucinol (Method B) to yield yellow coloured chromogen, exhibiting absorption maxima at 433nm and 421nm , respectively. Beer’s law was obeyed in the concentration ranges of 5-25 and 8-40µg/ml respectively. The coloured chromogen was stable for 4hrs. These methods were extended to pharmaceutical formulations and there was no interference from any common excepients which are usually present in tablet dosage formulations. The results of analysis have been validated statistically and by recovery studies.
KEYWORDS:
Mesalamine, Spectrophotometry, Chromotropic acid, phloroglucinol.
Cite:
Venugopal Darak, Arvind B. Karadi, Md. Arshad, S. Appal Raju. Spectrophotometric Determination of Mesalamine by Diazotization Coupling Reaction in Solid Dosage Forms. Asian J. Research Chem. 4(6): June, 2011; Page 968-970.
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