Journal :   Asian Journal of Research in Chemistry

Volume No. :   4

Issue No. :  6

Year :  2011

Pages :   971-975

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Extractive Spectrophotometric Methods for Determination of Pioglitazone Hydrochloride Using Acidic Triphenylmethane Dyes



Address:   M. Chennaiah1, T. Veeraiah2, T. Charan Singh1 and G. Venkateshwarlu1*
1Department of Chemistry, Nizam College (O.U), Hyderabad, 500001, India
2Department of Chemistry, SAP College Vikarabad, Ranga Reddy, A P, 501101, India
*Corresponding Author
DOI No:

ABSTRACT:
Three simple and sensitive extractive spectrophotometric methods have been described for the assay of Pioglitazone hydrochloride either in pure form or in pharmaceutical formulations. The developed methods involve formation of coloured chloroform extractable ion-pair complexes of the drug with bromothymol blue (BTB), bromophenol blue (BPB) and bromocresol purple (BCP) in acidic medium. The extracted complexes showed absorbance maxima at 415 nm for all three methods. Beer’s law is obeyed in the concentration ranges 2.0-30, 3.0-30 and 2.5-25µg/ml with BTB, BPB and BCP respectively. The effect of concentration of dye, pH, and interference of excipients have been studied and optimized. The limits of detection and quantification have been determined for three methods. All the three methods have been validated as per the guidelines of ICH. The methods have been applied to the determination of drug in commercial tablets and results of analysis were validated statistically through recovery studies.
KEYWORDS:
Pioglitazone hydrochloride; Bromothymol blue; Bromophenol blue; Bromocresol purple; Spectrophotometry.
Cite:
M. Chennaiah, T. Veeraiah, T. Charan Singh, G. Venkateshwarlu. Extractive Spectrophotometric Methods for Determination of Pioglitazone Hydrochloride Using Acidic Triphenylmethane Dyes. Asian J. Research Chem. 4(6): June, 2011; Page 971-975.
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