Journal :   Asian Journal of Research in Chemistry

Volume No. :   4

Issue No. :  6

Year :  2011

Pages :   1014-1018

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Production of L-Glutamic Acid by Immobilized Micrococcus Glutamicus Ab100.



Address:   S. Ganguly and A.K. Banik
Department of Chemical Engineering, Bio-chemical Engineering Division, Bio-Technology Laboratory, University of Calcutta, Kolkata – 700009, West Bengal, India.
*Corresponding Author
DOI No:

ABSTRACT:
Experimental studies were carried out to examine the efficiency of whole cell immobilization in calcium alginate beads and agar blocks for the production of L-glutamic acid using an auxotrophic mutant Micrococcus glutamicus AB100 developed in our laboratory by induced mutation from a regulatory mutant Micrococcus glutamicus AB1. Under the same physio-chemical environment, using selected suitable synthetic medium, different parameters for both calcium alginate beads and agar blocks were optimized one by one to maximize the production of L-glutamic acid. Production was increased with cells entrapped in calcium alginate beads significantly (p<0.05) compared to free cells with the presence of 0.1 (M) CaCl2 in the synthetic medium, 0.2 (M) CaCl2 for bead formation, 3.0% sodium alginate, 24h storage period of beads and 1.33 cell/alginate ratio but the accumulation of L-glutamic acid was decreased significantly (p<0.05) with the whole cell entrapped in agar blocks, compared to free cells with 4.0 mm3 agar block volume, 24h storage period of the blocks and 1.0 cell / agar ratio.
KEYWORDS:
Immobilization, mutant, calcium alginate, agar, L-glutamic acid.
Cite:
S. Ganguly, A.K. Banik. Production of L-Glutamic Acid by Immobilized Micrococcus Glutamicus Ab100. Asian J. Research Chem. 4(6): June, 2011; Page 1014-1018.
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