Journal :   Asian Journal of Research in Chemistry

Volume No. :   4

Issue No. :  8

Year :  2011

Pages :   1225-1230

ISSN Print :  0974-4169

ISSN Online :  0974-4150


Registration

Allready Registrered
Click to Login

Studies on DNA Extraction, Molecular Identification and Genetic Evolution of Trichoderma harzianum



Address:   1Senthilkumar G., 2Madhanraj P.* and 1Panneerselvam A.
1Dept of Botany and Microbiology, A. V. V. M Sri Pushpam College (Autonomous), Poondi, Thanjavur (Dt) - 613 503, Tamil Nadu, India.
2Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur - 621 212, Tamil Nadu, India.
*Corresponding Author
DOI No:

ABSTRACT:
The chromosomal DNA was isolated from T. harzianum and PCR amplification was performed. The 18S rDNA gene sequencing was made by using ITS (internal transcribed spacer) method. The 18S rDNA gene sequences were submitted to Genebank, NCBI, the accession number GU646678 has been obtained. The phylogenetic tree was constructed using MEGA 4 (Molecular Evolutionary Genetics Analysis Software version 4.0). Based on the neighbour joining tree analysis the isolate T. harzianum has been found closely related to already existing species of Hypocera tixii at 99 per cent of similarity.
KEYWORDS:
Trichoderma harzianum, DNA isolation, Evolution.
Cite:
Senthilkumar G., Madhanraj P., Panneerselvam A. Studies on DNA Extraction, Molecular Identification and Genetic Evolution of Trichoderma harzianum. Asian J. Research Chem. 4(8): August, 2011; Page 1225-1230.
[View HTML]      [View PDF]



Visitor's No. :   106482