Journal :   Asian Journal of Research in Chemistry

Volume No. :   4

Issue No. :  9

Year :  2011

Pages :   1371-1377

ISSN Print :  0974-4169

ISSN Online :  0974-4150


Registration

Allready Registrered
Click to Login

Method Development and Validation for Simultaneous Estimation of Metamizole Sodium and Pitofenone HCl by a Stability Indicating RP-HPLC



Address:   R. Vijayalakshmi and S. Anbazhagan
1Department of Pharmaceutical analysis, Gautham College of Pharmacy (Affiliated to Rajiv Gandhi University of Health Sciences), R.T. Nagar post, Bangalore, Karnataka -560032
2Karuna college of Pharmacy (Affiliated to Kerala University of Health Sciences), Irringuttur, Thirumittacode P.O. Palakad Dist. Kerala-679533.
*Corresponding Author
DOI No:

ABSTRACT:
A simple, selective, rapid and precise RP-HPLC has been developed for the simultaneous estimation of Metamizole sodium and Pitofenone HCl in presence of Fenpiverinium bromide of combined Pharmaceutical dosage forms. The Inertsil ODS 3V C-18 Column was used for Metamizole sodium and Pitofenone HCL separation. The Mobile phase used was sodium dihydrogen phosphate buffer : Methanol,(0.05M, pH 5.0), (53:47 v/v) at a flow rate of 1ml/min at 286nm. The calibration curves was linear at a concentration range of 700.90 µg/ml to1301.73 µg/ml and 7.01µg/ml to 13.01µg/ml with its regression coefficient (r2=0.9992 and 0.9995) for Metamizole sodium and Pitofenone HCl respectively was obtained. The LOD and LOQ was found to be in the range of 21.6174 µg/ml and 65.507 µg/ml for Metamizole sodium and 0.1701µg/ml and 0.5155 µg/ml for Pitofenone HCl respectively. The method is highly sensitive and successfully applied for determination of Metamizole sodium and Fenpiverinium bromide in tablet dosage forms.
KEYWORDS:
Metamizole sodium, Pitofenone HCl, Reverse phase High performance liquid chromatography.
Cite:
R. Vijayalakshmi, S. Anbazhagan. Method Development and Validation for Simultaneous Estimation of Metamizole Sodium and Pitofenone HCl by a Stability Indicating RP-HPLC. Asian J. Research Chem. 4(9): Sept, 2011; Page 1371-1377.
[View HTML]      [View PDF]



Visitor's No. :   113005