Journal :   Asian Journal of Research in Chemistry

Volume No. :   5

Issue No. :  11

Year :  2012

Pages :   1388-1391

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Studies on variation and stability of storage proteins and chymotrypsin inhibitors of an underutilized legume, Mucuna during germination



Address:   Kempohalli S. Chandrashekharaiah*
Department of Biotechnology, PES Institute of Technology, BSK III stage, Bangalore-560085, Karnataka, India.
*Corresponding Author
DOI No:

ABSTRACT:
The proteins and chymotrypsin inhibitors were isolated and studied from the seeds of eight varieties of Mucuna. The total protein content varied from 15 – 26%. The seeds of all the varieties of Mucuna exhibited chymotrypsin inhibitor activity, varied from 3 – 7 CIA/mg of protein. There was no much variation in the chynotrypsin inhibitory activity among soaked seeds and dry seeds. The Mucuna seeds were germinated for six days and change in the total protein content and chymotrypsin inhibitors were monitored. The total protein content and chymotrypsin inhibitory activity of the endosperm showed reciprocal variation, such that, the protein content increased up to 72 hrs of germination and then decreased followed by decreased chymotrypsin inhibitory activity with increase in germination time. The chymotrypsin inhibitor activity was decreased from 6.78 CIA/mg to 1.24 CIA/mg (81% CIA reduction after 144 hrs germination). The proteins were numbered from anodic end. The protein banding pattern revealed the presence of total 11 bands on anionic polyacrylamide gel electrophoresis. Mucuna cochinensis and Mucuna cochinensis MP9 were identical in both number of bands (total of 09 bands) and banding pattern. Electrophoretic analysis of chymotrypsin inhibitors revealed the presence of five to seven isoinhibitors in the seeds of different varieties of Mucuna. The Mucuna hirsute and Mucuna pruriens contained a maximum of seven chymotrypsin isoinhibitors.
KEYWORDS:
Mucuna seeds, proteins, chymotrypsin inhibitors, germination.
Cite:
Kempohalli S. Chandrashekharaiah. Studies on variation and stability of storage proteins and chymotrypsin inhibitors of an underutilized legume, Mucuna during germination. Asian J. Research Chem. 5(11): Nov., 2012; Page 1388-1391.
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