Journal :   Asian Journal of Research in Chemistry

Volume No. :   5

Issue No. :  11

Year :  2012

Pages :   1409-1412

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Development and Validation of a Stability-Indicating RP-UPLC Method for Assay of 1-(4-Hydrozinophenyl) methyl-1,2,4-triazole Dihydrochloride and Estimation of its Related Compounds



Address:   P. Srinivas1*, T Siva Rao1, Mohammad Younus2, Elaiyalvar Ramakrishnan3, B Chaitanya4
1Dept. of Inorganic Analytical Chemistry, Andhra University, Vishakhapatnam, Andhra Pradesh, India
2 Research and Development Dept. Chandra Labs, Hyderabad, Andhra Pradesh, India
3 Dept of Pharmaceutical Chemistry, Manipal College of Pharmaceutical Sciences, Karnataka, India
4 Dept. of Chemistry, P.R College, Bharathiar University, Tamil Nadu, India
*Corresponding Author
DOI No:

ABSTRACT:
1-(4-Hydrozinophenyl) methyl-1, 2, 4-triazole dihydrochloride (HPT) is an intermediate for synthesis of Rizatriptan. A stability indicating Reverse Phase Ultra High Pressure Liquid Chromatographic (RP-UPLC) method has been developed for the determination of 1-(4-Hydrozinophenyl) methyl-1, 2, 4-triazole Dihydrochloride and its related compounds. The stability-indicating capability of this method has been demonstrated by adequate separation of the degradation products from HPT in stress degraded samples. The separation was achieved on kinetex 1.7 XB-C 18 100 A 50 X 2.1 mm column. The gradient mobile phase consisted mobile phase A 0.01 M potassium dihydrogen phosphate buffer pH 4.5 (adjusted with Orthophosphoric acid) and mobile phase B acetonitrile & methanol in the ratio 80: 20. The method has been demonstrated to be specific, accurate, linear, precise, robust and therefore suitable for routine analysis of HPT.
KEYWORDS:
1-(4-Hydrozinophenyl)methyl-1,2,4-triazole Dihydrochloride, rizatriptan, Reverse Phase Ultra High Pressure Liquid Chromatography, related compounds.
Cite:
P. Srinivas, T Siva Rao, Mohammad Younus, Elaiyalvar Ramakrishnan, B Chaitanya. Development and Validation of a Stability-Indicating RP-UPLC Method for Assay of 1-(4-Hydrozinophenyl) methyl-1,2,4-triazole Dihydrochloride and Estimation of its Related Compounds. Asian J. Research Chem. 5(11): Nov., 2012; Page 1409-1412.
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