Journal :   Asian Journal of Research in Chemistry

Volume No. :   5

Issue No. :  12

Year :  2012

Pages :   1415-1418

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Standardization of ‘Avipathikara Churna’: A HPLC Approach



Address:   Dubey Darshan* and Dashora Kamlesh
Institute of Pharmacy, Vikram University, Ujjain (M.P.) 456001, India
*Corresponding Author
DOI No:

ABSTRACT:
Quantification of active principles through modern analytical tools is necessary for establishing the safety, quality, acceptability and usage of herbal formulations. ‘Avipathikara churna’ is one of the oldest and popular Ayurvedic preparations, is official in Ayurvedic formulary of India used widely for disorder of Digestive impairment, Constipation, Dyspepsia, Haemorrhoids, Retention of urine and Urinary disorders. It comprised of the fruits Piper longum (Pippali), Piper nigrum (Marica), Embelica ribes (Vidanga) rhizomes of Zingiber officianalis (Saunth), Cyperus rotundus (Musta) pericarp of Terminelia chebula (Haritaki), Terminelia bellirica (Babhitaka), Embelica officinalis (Amalaki) seed of Elettaria cardamomum (Ela), leaf of Cinnamomum tamala (Tejpatra), Flower bud of Eugenia caryuphyllus (Lavang) and Operculina turpethum (Trivrt).The present study is an attempt to develop the fingerprint method for Avipathikara Churna by simple high-performance liquid chromatography (HPLC) determination using Gallic Acid as a standard, which is as an important and major content in formulation. RP- HPLC methods for determination of Galiic Acid in Vidanga, Haritaki, Babhitaka, Musta and Amalaki have been developed. A C 18 LUNA (5 micron 25 cm×4.6 mm) column from Phenomenex in binary gradient mode with mobile phase acetonitrile: ethyl acetate at flow rate is 1.2ml/min, and effluent was monitored at 264 nm. Validation of the method was done with a view to demonstrate its selectivity, linearity, precision and accuracy. The concentration of gallic acid present in raw material was found to be 3.096 ±0.729w/w in amalaki, 7.612±0.289 in babhitaka 4.658±0.902 in haritaki 0.211%±0.783 in musta and 0.967%±0.439 w/w in vidanga respectively and in three identical laboratory batch of Avipathikara Churna name AP-I, AP-II, AP-III, was 0.136±0.482, 0.135±0.708, 0.136±0.604w/w respectively. The Gallic acid content of all the three batches is found to be in close proximities with each other. Obtained results were compared with marketed formulation.
KEYWORDS:
Gallic acid, Avipathikara Churna, HPLC, Ayurvedic Formulation, Fingerprinting.
Cite:
Dubey Darshan, Dashora Kamlesh. Standardization of ‘Avipathikara Churna’: A HPLC Approach. Asian J. Research Chem. 5(12): Dec., 2012; Page 1415-1418.
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