Journal :   Asian Journal of Research in Chemistry

Volume No. :   5

Issue No. :  2

Year :  2012

Pages :   221-224

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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RP-HPLC Method Development and Validation for the Estimation of Cinitapride and Pantoprazole in Solid Oral Dosage Form



Address:   P.V. Hemalatha, A. Jerad Suresh and Niraimathi V.*
Department of Pharmaceutical Chemistry, College of Pharmacy, Madras Medical College, Chennai-600003, Tamil Nadu, India.
*Corresponding Author
DOI No:

ABSTRACT:
The present study involves the development of a simple, specific, accurate, rapid and cost effective RP-HPLC method assisted with UV detection for the estimation of Cinitapride (CNP) and Pantoprazole (PNP) in solid oral dosage forms. The method utilized C18 column (250x4.6 i.d 5µ particle size) and a mobile phase consisting of 40:20:40 ratio of acetonitrile: methanol: phosphate buffer (pH 7). CNP was detected at 262 and PNP at 290nm with a retention time of 6.5 and 3.5minutes respectively. The chromatographic condition and polarity of the mobile phase were optimized. The drugs showed good linearity, CNP at 84-132µg/ml and PNP at 140-220µg/ml with a correlation coefficient equal to 1. The recovery percentage were found to be 99-100%. Thus the proposed method is simple, fast, accurate and could be applied for the routine analysis of CNP and PNP in pure and pharmaceutical dosage forms.
KEYWORDS:
, Pantoprazole, RP-HPLC.
Cite:
P.V. Hemalatha, A. Jerad Suresh, Niraimathi V.. RP-HPLC Method Development and Validation for the Estimation of Cinitapride and Pantoprazole in Solid Oral Dosage Form. Asian J. Research Chem. 5(2): February 2012; Page 221-224.
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