Journal :   Asian Journal of Research in Chemistry

Volume No. :   5

Issue No. :  2

Year :  2012

Pages :   193-196

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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HPTLC methods for quantification of gallic acid in Bhuvneshvara vati for routine quality control



Address:   V. Jain1*, S.J. Daharwal1, Tripti Jain2, Swarnlata Saraf1and S. Saraf1
1University Institute of Pharmacy, Pt. Ravi Shankar Shukla University, Raipur (C.G.), 492 010
2Chhattisgarh Food and Drug Administration, Raipur (C.G.) 492 010
*Corresponding author
DOI No:

ABSTRACT:
Bhuvneshvara vati is one of the popular Ayurvedic preparations, official in Ayurvedic formulary of India. The present study is an attempt to develop a fingerprint method for Bhuvneshvara vati HPTLC with Method using gallic acid as a standard. The gallic acid is a major content in all the five herbal ingredients of the formulation. The method was validated for linearity, accuracy, limit of detection, limit of quantification, inter-day and intra-day assay precision, repeatability of measurement, and repeatability of sample application. The concentration of gallic acid present in raw material was found to be 3.10 ±0.41% w/w in emblica officinalis, 8.47±0.82% w/w in terminalia belerica, 4.63±0.49% w/w in Terminalia chebula, 1.82±0.73% w/w in Aegle marmelous and 0.26±0.65% w/w in Trichyspermum ammi. Gallic acid content in three identical laboratory batch of Bhuvneshvara vati BV-I, BV-II and BV-III, is found to be 2.61±0.468%, 2.59±0.567% and 2.63±0.392% w/w respectively with mean value 2.61±0.476%. The gallic acid content in all the three different batches is found to be in close proximities with each other.The results were comparable to marketed formulations.
KEYWORDS:
HPTLC, Gallic acid, Bhuvneshvara vati , Ayurvedic formulation, Fingerprinting.
Cite:
V. Jain, S.J. Daharwal, Tripti Jain, Swarnlata Saraf, S. Saraf. HPTLC methods for quantification of gallic acid in Bhuvneshvara vati for routine quality control. Asian J. Research Chem. 5(2): February 2012; Page 193-196.
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