Journal :   Asian Journal of Research in Chemistry

Volume No. :   5

Issue No. :  3

Year :  2012

Pages :   409-413

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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In-Vitro Antimicrobial Potency and Pharmacological Study of Flower extracts in different polar solvents of Withania somnifera



Address:   Dr. Premlata Singariya1*, Dr. Padma Kumar1 and Dr. Krishan Kumar Mourya2.
1Dr. D.S. Kothari Post Doctoral Fellow, Laboratory of Tissue Culture and Secondary Metabolites, Department of Botany, University of Rajasthan, Jaipur- 302004
2Veterinary Officer and In-charge, Ist Gr. Govt. Veterinary Hospital, Pahari (Bharatpur) Rajasthan.
*Corresponding Author
DOI No:

ABSTRACT:
The flower of Withania somnifera were evaluated against medically important bacteria viz. Proteus merabilis, Klebsiella pnemoniae, Agerobacterium tumefaciens (plant pathogen) and one fungi Aspergillus niger. The dried and powdered flowers were successively extracted with a series of non polar to polar solvents using soxhlet assembly. The antimicrobial assay was done by both disc diffusion assay (DDA) and broath dilution methods. Glacial acetic acid extract and iso propyl extract of W. somnifera show highest activity against A. tumefaciens (plant pathogen) to varying degrees, by most of the extracts. Gentamycin, the standard antibacterial drug used was effective in inhibiting these bacteria. The effect on A. tumefaciens and K. pneumoniae were comparable to that of gentamycin. Ketoconazole the standard anti fungi used was effective against the fungi. The extract of W. somnifera also significantly (P>0.005) inhibited the bacterial and fungal growth. The inhibitory effect is very identical in magnitude and comparable with that of standard antibiotics used.
KEYWORDS:
Solanacae, Antibacterial, antifungal, Disc diffusion assay and broth dilution method.
Cite:
Premlata Singariya, Padma Kumar, Krishan Kumar Mourya. In-Vitro Antimicrobial Potency and Pharmacological Study of Flower extracts in different polar solvents of Withania somnifera. Asian J. Research Chem. 5(3): March 2012; Page 409-413.
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