Journal :   Asian Journal of Research in Chemistry

Volume No. :   5

Issue No. :  3

Year :  2012

Pages :   340-344

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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A new LCMS/MS method for estimation of Bupropion and its metabolite in human plasma

Address:   G. Ramu1, A. K. Meher1, P. Anandan1, S.G. Pampalia2 and A. Mukhopadhyay1*
1Bioanalytical Laboratories, LCRA (A Division of Norwich Clinical Services Pvt. Ltd.), 147/F, 8th Main, 3rd Block, Koramangala, Bangalore -560 034
2Birla Institute of Technology, Mesra, Ranchi - 835215, Jharkhand.
*Corresponding Author

Bupropion, hydroxybupropion and fluoxetine, (IS) were extracted from the plasma by LLE method and then separated on a Zorbax SB C8 column at 500C using the mobile phase mixture of 0.2% formic acid and methanol (35:65, v/v) at a flow rate of 1.0 ml/min. The analytes were detected in API 3000 Mass spectrometer in the positive electrospray ionization mode (split ratio 1.5:1) with multiple reactions monitoring (MRM). The MRM transitions were monitored by following m/z for parent ion 240.1 and daughter ion 184.1 (Bupropion), m/z 256.0 and daughter 238.0 (Hydroxybupropion) and m/z 310.0 and daughter 44.0 (Fluoxetine). A linear calibration plot of bupropion was achieved in the concentration ranges of 1.949 to 270.665 ng/ml and for hydroxybupropion, it was 5.152 to 715.497 ng/ml. Recoveries were consistently more than 60.0%. The assay was specific, precise, accurate and reproducible.
Bupropion, Hydroxybupropion, LCMS/MS, Human plasma, Validation.
G. Ramu, A. K. Meher, P. Anandan, S.G. Pampalia, A. Mukhopadhyay. A new LCMS/MS method for estimation of Bupropion and its metabolite in human plasma. Asian J. Research Chem. 5(3): March 2012; Page 340-344.
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