Journal :   Asian Journal of Research in Chemistry

Volume No. :   5

Issue No. :  4

Year :  2012

Pages :   515-519

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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Validated RP-HPLC Method for Estimation of Rasagiline in Tablet Dosage Form



Address:   Bhavin Vaishnani, Ritu Kimbahune, Prachi Kabra*, Sanjay Surani, L.V.G. Nargund
Department of Quality Assurance, Nargund College of Pharmacy, Dattatreyanagar, II Main,100 Ft Ring Road, BSK III Stage, Bangalore- 560 085, India.
*Corresponding Author
DOI No:

ABSTRACT:
In the present study, a reverse phase high performance liquid chromatographic method was developed and validated for the determination of Rasagiline in tablet dosage form with the use of Caffeine as an internal standard. Chromatographic separation was carried out on a RP-18 column using a mobile phase consisting of acetonitrile: isopropyl alcohol: water (10:20:70, v/v/v) adjusted at pH 2.5 with o-phosphoric acid (1%). The flow rate was maintained at 0.8 ml min-1 and UV detection was measured at 210 nm. The calibration curve was linear over the range 1-200µgml-1. R.S.D. for precision was <3%. The results of accuracy study was observed in the range of 95.16% to 102.24 % with R.S.D. < 3%. LOD and LOQ were found to be 0.4044µgml-1 and 1.2133µgml-1, respectively. The method was simple, rapid, and easy to apply, making it suitable for routine analysis of Rasagiline in tablet dosage form.
KEYWORDS:
Rasagiline, Caffeine, RP-HPLC, Validation.
Cite:
Bhavin Vaishnani, Ritu Kimbahune, Prachi Kabra, Sanjay Surani, L.V.G. Nargund. Validated RP-HPLC Method for Estimation of Rasagiline in Tablet Dosage Form. Asian J. Research Chem. 5(4): April 2012; Page 515-519.
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