Journal :   Asian Journal of Research in Chemistry

Volume No. :   5

Issue No. :  4

Year :  2012

Pages :   472-476

ISSN Print :  0974-4169

ISSN Online :  0974-4150


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RP-HPLC Method Development and Validation for Simultaneous Estimation of Irbesartan and Hydrochlorothiazide in Pharmaceutical Dosage Form



Address:   M.M. Eswarudu1*, T. Narendra Chary1, Sunil Junapudi1, M. Sushma2.
1.Department of pharmaceutical analysis, Anurag pharmacy college, Ananthagiri(V), Kodad(M), Nalgonda (Dt), Andhra Pradesh, India.508206
2.D.C.R.M. Pharmacy College, Inkollu, Prakasham Dist, Andhra Pradesh, India.
*Corresponding Author
DOI No:

ABSTRACT:
A simple, reproducible and efficient high performance liquid chromatographic method was developed for simultaneous determination of Irbesartan and Hydrochlorothiazide in tablets. A Hypersil pack BDS C18 column 250X4.6 mm 5µ particle size in isocratic mode with mobile phase containing acetonitrile: Buffer (sodium acetate anhydrous) (55:45 v/v ) adjusted to pH 3.5 using ortho phosphoric acid. The flow rate was 1.0 ml/min and effluent was monitored at 260 nm. The retention time and linearity range for Irbesartan and Hydrochlorothiazide were (2.98, 4.83 min) and (5-200, 1-400 µg/ml), respectively. The developed method was found to be accurate, precise and selective for simultaneous determination of Irbesartan and Hydrochlorothiazide in tablet dosage form.
KEYWORDS:
Irbesartan, Hydrochlorothiazide, RP-HPLC, simultaneous determination, Validation.
Cite:
M.M. Eswarudu, T. Narendra Chary, Sunil Junapudi, M. Sushma. RP-HPLC Method Development and Validation for Simultaneous Estimation of Irbesartan and Hydrochlorothiazide in Pharmaceutical Dosage Form. Asian J. Research Chem. 5(4): April 2012; Page 472-476.
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